Use of dual priming oligonucleotide system-based multiplex RT-PCR assay to detect five diarrhea viruses in pig herds in South China

AMB Express

Table 1 Details of the conventional primers and the DPO primers used in this study

Virus	Gene	Type	Sequence (5′→3′)	Amplicon
TGEV	N	C^a	F^c: GGTGGTTCTTCTACTACTTAGGTACTGGACCTCATG	472
		C^a	R^d: ACATCACCTTTACCTGCAGTTCTCTTCCAGGTGTG
		DPO^b	F: GGTGGTTCTTCTACTACTTAGGIIIII^eGACCTCATG
		DPO^b	R: ACATCACCTTTACCTGCAGTTIIIIICCAGGTGTG
PEDV	N	C	F: GGTGAGCGAATTGAACAACCTTCCAATTGGCATTTC	361
		C	R: GACCCTGGTTATTTCCACGATTCTGTGAATTACCAC
		DPO	F: GGTGAGCGAATTGAACAACCTTCIIIIIGGCATTTC
		DPO	R: GACCCTGGTTATTTCCACGATTCTIIIIITTACCAC
PRV-A	VP7	C	F: CTCCTTTTAATGTATGGTATTGAATATACCACAG	123
		C	R: AAGAAATCTATAAATTATAAAGTCCATCGCACTAG
		DPO	F: CTCCTTTTAATGTATGGTATTIIIIITACCACAG
		DPO	R: AAGAAATCTATAAATTATAAAGIIIIICGCACTAG
PDCoV	N	C	F: TCAACGCTAGAGGAAGACCTCAGGAGCGTGGAAGTG	263
		C	R: CATGATGCGAGGATCAGCCATACCCGTCTTCTCAG
		DPO	F: TCAACGCTAGAGGAAGACCTCAGIIIIITGGAAGTG
		DPO	R: CATGATGCGAGGATCAGCCATAIIIIICTTCTCAG
SADS-CoV	N	C	F: GACCTGACTGTTGTTGAGGTTACTTCTAGAAGTGC	586
		C	R: TCCTAATTTGACTGGGTTTAGAGTAAGCCGAGACT
		DPO	F: GACCTGACTGTTGTTGAGGTTAIIIIIAGAAGTGC
		DPO	R: TCCTAATTTGACTGGGTTTAGAIIIIICCGAGACT