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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Use of dual priming oligonucleotide system-based multiplex RT-PCR assay to detect five diarrhea viruses in pig herds in South China

Fig. 3

Annealing temperature optimization comparison between DPO primers and conventional primers. Lanes 1–6 represent 40 °C, 44 °C, 48 °C, 52 °C, 56 °C, 60 °C, for a gradient with 6 temperatures; Lane 7 represents negative template (N). a DPO primer annealing temperature optimization test; b Normal annealing temperature optimization test for primers

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