A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

AMB Express

Table 3 Activity comparison of recombinant bacterial α-N-acetylglucosaminidases in catalyzing the cleavage of GlcNAcα1–4GlcAβProNHFmoc (2) at pH 6.5 and 37 °C

Catalyst	Concentration of catalyst (mM)	Conversion (%)
Catalyst	Concentration of catalyst (mM)	1 h	20 h
CpGH89	0.11	13.9 ± 0.7	96.0 ± 0.4
tCpGH89	0.11	23.7 ± 1.9	100
∆17Bf0576	0.15	66.9 ± 0.6	100
∆24Bt0438	0.036	12.2 ± 0.8	91.4 ± 0.6
∆22Bt3590	0.003	42.9 ± 1.2	97.0 ± 0.4