Skip to main content

Table 3 Activity comparison of recombinant bacterial α-N-acetylglucosaminidases in catalyzing the cleavage of GlcNAcα1–4GlcAβProNHFmoc (2) at pH 6.5 and 37 °C

From: A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

Catalyst Concentration of catalyst (mM) Conversion (%)
1 h 20 h
CpGH89 0.11 13.9 ± 0.7 96.0 ± 0.4
tCpGH89 0.11 23.7 ± 1.9 100
∆17Bf0576 0.15 66.9 ± 0.6 100
∆24Bt0438 0.036 12.2 ± 0.8 91.4 ± 0.6
∆22Bt3590 0.003 42.9 ± 1.2 97.0 ± 0.4