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AMB Express

Table 2 Activity comparison of recombinant bacterial α-N-acetylglucosaminidases in catalyzing the cleavage of GlcNAcαMU (1) at pH 6.5 and 37 °C

From: A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

Enzyme Concentration of catalyst (μM) Conversion (%)
20 min 20 h
CpGH89 0.38 25.2 ± 0.4 100
tCpGH89 0.38 24.9 ± 0.4
∆17Bf0576 0.49 25.5 ± 1.5
∆24Bt0438 0.048 36.6 ± 1.8
∆22Bt3590 0.012 38.7 ± 0.3
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