A GH89 human α-N-acetylglucosaminidase (hNAGLU) homologue from gut microbe Bacteroides thetaiotaomicron capable of hydrolyzing heparosan oligosaccharides

AMB Express

Table 2 Activity comparison of recombinant bacterial α-N-acetylglucosaminidases in catalyzing the cleavage of GlcNAcαMU (1) at pH 6.5 and 37 °C

Enzyme	Concentration of catalyst (μM)	Conversion (%)
Enzyme	Concentration of catalyst (μM)	20 min	20 h
CpGH89	0.38	25.2 ± 0.4	100
tCpGH89	0.38	24.9 ± 0.4
∆17Bf0576	0.49	25.5 ± 1.5
∆24Bt0438	0.048	36.6 ± 1.8
∆22Bt3590	0.012	38.7 ± 0.3