Identification of the molecular mechanisms underlying brisket disease in Holstein heifers via microbiota and metabolome analyses

AMB Express

Table 4 Differential metabolites were identified in BD by mass spectrometry

MS2.name	VIP	P-value	Log₂(fold change)
Beta-d-fructose	1.92	< 0.001	− 2.89
d-ribose	2.25	0.015	− 1.14
1,4-beta-d_glucan	1.18	0.019	− 1.20
Sucrose	1.12	0.002	− 1.09
Glucose-6-phosphate	1.25	0.014	− 1.25
l-Asparagine	1.03	0.010	− 0.83
Ala-Gly	2.13	0.012	− 2.90
His-Ile	2.00	0.004	− 2.65
Lys-Phe	2.05	0.017	3.62
l-leucyl-l-proline	2.28	0.004	4.07
Glycyl-l-leucine	1.99	< 0.001	− 3.29
l-Cysteine	1.12	0.033	1.09

VIP variable important for the projection, MS mass spectrometry, BD brisket disease, HH healthy heifer