Table 1 Bacterial strains and plasmids used in this study

Strains

E. coli

E. coli DH5α

F⁻, Φ80d/lacZΔM15, Δ (lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(r_k⁻m_k⁺), phoA, supE44, λ⁻, thi-1, gyrA96, relA

TaKaRa

E. coli str. K-12 substr. W3110

Wild strain

ND

C. glutamicum

C. glutamicum ATCC 13032

Wild strain

ND

C. glutamicum ATCC 14067

Wild strain

ND

C. glutamicum ssp. lactofermentum

A strain of C. glutamicum for l-isoleucine production

Shi et al. (2019)

CGN001

C. glutamicum ATCC 13032, Δldh

This study

CGN002

C. glutamicum ATCC 13032, ΔeutD

This study

CGN003

C. glutamicum ATCC 13032, ΔgabP

This study

CGN004

C. glutamicum ATCC 13032, ΔglnA1

This study

CGN005

C. glutamicum ATCC 13032, ΔglnA2

This study

CGN006

C. glutamicum ATCC 13032, ΔalaT

This study

CGN007

C. glutamicum ATCC 13032, ΔargR

This study

CGN008

C. glutamicum ATCC 13032, ΔgabTD

This study

CGN009

C. glutamicum ATCC 13032, ΔaceAB

This study

CGN010

C. glutamicum ATCC 13032, ΔpoxB

This study

CGG001

C. glutamicum ATCC 13032, Φ(PtacM-gdhA1)

This study

CGG002

CGG1, ΔgabP::gadB

This study

CGG003

C. glutamicum ATCC 13032, ΔgabP::gadB

This study

CGG004

CGG003, ΔeutD::gdhA

This study

CGG005

CGG004, ΔgabTD

This study

CGC001

C. glutamicum ATCC 13032, ΔeutD::speC

This study

CGY001

C. glutamicum ATCC 14067, ΔgabP

This study

CGY002

C. glutamicum ssp. lactofermentum, ΔgabP

This study

Plasmids

pDTW109

Temperature-sensitive plasmid in C. glutamicum, Cm^r

Hu et al. (2013)

pFSC

A plasmid harboring codon-optimized cas9 gene, kan^r

Peng et al. (2017)

pBluescript II SK

A plasmid containing PUC ori replicon, amp^r

Hu et al. (2013)

pEC-XK99E

E. coli—C. glutamicum shuttle plasmid, kan^r

Hu et al. (2014)

pJYW-5-gdhA

A recombination plasmid harboring gdhA under promoter PtacM, kan^r

This study

pJYW-5-speC

A recombination plasmid harboring speC under promoter PtacM, kan^r

This study

pJYW-5-gadB

A recombination plasmid harboring gadB under promoter PtacM, kan^r

This study

pJYW-5-gdhA1

A recombination plasmid harboring gdhA1 under promoter PtacM, kan^r

This study

pCCG1

E. coli—C. glutamicum shuttle plasmid, CRISPR/cas9 vector, pBL1^TS, kan^r

This study

pCCG2

A vector derive from pCCG1, pBL1^TS, kan^r

This study

pBS-sgRNA

pBluescript II SK harboring sgRNA sequence

This study

pCCG1-ldh

pCCG1 harboring ldh deletion elements, kan^r

This study

pCCG1-eutD

pCCG1 harboring eutD deletion elements, kan^r

This study

pCCG1-gabP

pCCG1 harboring gabP deletion elements, kan^r

This study

pCCG1-glnA1

pCCG1 harboring glnA1 deletion elements, kan^r

This study

pCCG1-glnA2

pCCG1 harboring glnA2 deletion elements, kan^r

This study

pCCG1-alaT

pCCG1 harboring alaT deletion elements, kan^r

This study

pCCG1-argR

pCCG1 harboring argR deletion elements, kan^r

This study

pCCG1-gabTD

pCCG1 harboring gabTD deletion elements, kan^r

This study

pCCG1-aceAB

pCCG1 harboring aceAB deletion elements, kan^r

This study

pCCG1-poxB

pCCG1 harboring poxB deletion elements, kan^r

This study

pCCG1-PtacM-gdhA1

The plasmid to replace gdhA1 native promoter with PtacM and artificial RBS, kan^r

This study

pCCG1-ΔalaT::gdhA

The plasmid to insert gdhA into alaT, kan^r

This study

pCCG2-ΔalaT::gdhA

The plasmid to insert gdhA into alaT, kan^r

This study

pCCG1-ΔeutD::speC

The plasmid to insert speC into eutD, kan^r

This study

pCCG1-ΔgabP::gadB

The plasmid to insert gadB into gabP, kan^r

This study

pCCG1-ΔgabP::gadB2-gadB1^m

The plasmid to insert cluster gadB2B1^m into gabP, kan^r

This study

pCCG1-ΔeutD::gdhA

The plasmid to insert gdhA into eutD, kan^r

This study

pCCG1-gabP (14067)

pCCG1 harboring gabP deletion elements in ATCC 14067, kan^r

This study

pCCG1-gabP (ssp. lactofermentum)

pCCG1 harboring gabP deletion elements in ssp. lactofermentum, kan^r

This study