Table 1 Strains and plasmids used in this study
Strain or plasmid | Description | Source or reference |
|---|---|---|
Strains | ||
Escherichia coli | ||
HST08 | Cloning host | TaKaRa |
TOP10 | Modification host | Invitrogen |
M. thermoacetica | ||
ATCC 39073 | Wild-type strain | ATCC |
ΔpyrF | pyrF gene was deleted | Kita et al. (2013) |
pyrF::acetone | The thermophilic acetone operon was introduced into the pyrF region by using pHM17 | This study |
pduL2::acetone | The thermophilic acetone operon was introduced into the pduL2 region by using pHM5. As a result, pduL2 was knocked out | This study |
Plasmids | ||
pBAD33 | Backbone plasmid for methylation plasmids, Cmr | Guzman et al. (1995) |
pBAD-M1281 | pBAD33 carrying the Moth_1671, Moth_1672, and Moth_2281 for DNA methylation | Kita et al. (2013) |
pK18mob | Backbone plasmid for transformation plasmids, Kmr | Schäfer et al. (1994) |
pK18-ldh | A transformation plasmid to introduce ldh into the pyrF region by using pyrF as a selection marker | Kita et al. (2013) |
pK18-ΔpduL2::ldh | A transformation plasmid to introduce ldh into the pduL2 region by using pyrF as a selection marker | Iwasaki et al. (2017) |
pHM17 | A transformation plasmid to introduce the thermophilic acetone operon into the pyrF region by using pyrF as a selection marker | This study |
pHM5 | A transformation plasmid to introduce the thermophilic acetone operon into the pduL2 region by using pyrF as a selection marker | This study |