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Table 1 Strains and plasmids used in this study

From: Metabolic engineering of Moorella thermoacetica for thermophilic bioconversion of gaseous substrates to a volatile chemical

Strain or plasmid Description Source or reference
Strains
Escherichia coli
 HST08 Cloning host TaKaRa
 TOP10 Modification host Invitrogen
M. thermoacetica
 ATCC 39073 Wild-type strain ATCC
 ΔpyrF pyrF gene was deleted Kita et al. (2013)
 pyrF::acetone The thermophilic acetone operon was introduced into the pyrF region by using pHM17 This study
 pduL2::acetone The thermophilic acetone operon was introduced into the pduL2 region by using pHM5. As a result, pduL2 was knocked out This study
Plasmids
 pBAD33 Backbone plasmid for methylation plasmids, Cmr Guzman et al. (1995)
 pBAD-M1281 pBAD33 carrying the Moth_1671, Moth_1672, and Moth_2281 for DNA methylation Kita et al. (2013)
 pK18mob Backbone plasmid for transformation plasmids, Kmr Schäfer et al. (1994)
 pK18-ldh A transformation plasmid to introduce ldh into the pyrF region by using pyrF as a selection marker Kita et al. (2013)
 pK18-ΔpduL2::ldh A transformation plasmid to introduce ldh into the pduL2 region by using pyrF as a selection marker Iwasaki et al. (2017)
 pHM17 A transformation plasmid to introduce the thermophilic acetone operon into the pyrF region by using pyrF as a selection marker This study
 pHM5 A transformation plasmid to introduce the thermophilic acetone operon into the pduL2 region by using pyrF as a selection marker This study