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Table 3 Comparison of different C. glutamicum strains for CGP synthesis

From: Incorporation of alternative amino acids into cyanophycin by different cyanophycin synthetases heterologously expressed in Corynebacterium glutamicum

Strains DW [g/l] Cyanophycin
   Solubility CGP content [%DW] Amino acid composition[mol%] *
Asp Arg Lys Glu Orn Cit
ATCC 13032
 pVWEx1::cphA6308Δ1 15.9 ± 0.1 Insoluble 1.9 ± 0.5 50.1 38.9 8.7 0.5 1.0 0.8
Soluble 4.0 ± 0.1 51.9 26.9 10.9 1.2 6.6 2.5
 pVWEx1::cphA6308Δ1_C595S 15.6 ± 0.2 Insoluble 1.4 ± 0.3 54.7 30.0 13.6 0.3 0.8 0.6
Soluble 8.3 ± 2.9 51.3 27.5 10.7 0.9 6.4 3.2
 pVWEx1::cphADh 16.6 ± 0.4 Insoluble 0.0 ± 0.0
Soluble 36.2 ± 0.7 49.3 40.1 6.9 2.3 0.6 0.8
DM1279
 pVWEx1::cphA6308Δ1 11.6 ± 0.3 Insoluble 12.2 ± 2.1 52.9 30.3 16.2 0.2 0.0 0.4
Soluble 2.9 ± 0.6 45.6 21.0 29.7 1.0 2.2 0.5
pVWEx1::cphA6308Δ1_C595S 11.7 ± 0.2 Insoluble 13.1 ± 2.3 48.0 29.8 21.6 0.2 0.0 0.4
Soluble 2.1 ± 0.5 42.7 19.8 33.5 1.0 2.6 0.4
 pVWEx1::cphADh 14.4 ± 0.6 Insoluble 0.0 ± 0.0
Soluble 24.2 ± 1.9 48.3 33.3 15.4 2.2 0.8 0.0
ORN2(Pgdh4)
 pVWEx1::cphA6308Δ1 11.6 ± 0.2 Insoluble 1.9 ± 0.2 47.6 42.7 0.0 0.3 9.1 0.3
Soluble 3.2 ± 0.7 46.0 43.5 0.0 0.6 9.4 0.5
 pVWEx1::cphA6308Δ1_C595S 11.7 ± 0.1 Insoluble 2.4 ± 0.2 42.8 47.9 0.0 0.3 8.7 0.3
Soluble 2.3 ± 0.7 41.7 44.2 0.0 0.7 12.6 0.8
 pVWEx1::cphADh 12.9 ± 0.3 Insoluble 0.0 ± 0.0
Soluble 18.6 ± 1.6 42.8 43.7 0.0 1.4 11.7 0.4
  1. The strains were cultivated for 72 h in 100 ml CGXII medium in a 500 ml Erlenmeyer flask with two baffles at 130 rpm and 30 °C. The cultures were inoculated with an OD600 of about 0.2 and after 4 h IPTG was added. For the ORN2(Pgdh4) strains an additional 15 mM arginine was added to the medium. The cells dry mass was determined, and the contained cyanophycin was isolated and analysed. The values indicate the mean and standard deviation of at least three independent cultivations
  2. * The values have been rounded to one decimal place