Table 1 The parameters of purification of serratiopeptidase from culture supernatant up to ion-exchange chromatography
From: Purification and characterization of thermoactive serratiopeptidase from Serratia marcescens AD-W2
Sample | Total protease unit | Total protein content (mg) | Specific activity (units of enzyme activity/mg protein) | Purification fold |
|---|---|---|---|---|
Culture supernatant | 613,851.8 | 158.3 | 3877.466 | 1 |
Ammonium sulphate (40–80%) | 49,156.85 | 6.42 | 7658.901 | 1.97 |
Ion exchange chromatography (MonoQ column) | 39,533.85 | 1.93 | 20,492.59 | 5.28 |