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Table 2 Plackett–Burman design: experimental procedure, variables and statistical analysis

From: Orchestration an extracellular lipase production from Aspergillus niger MYA 135: biomass morphology and fungal physiology

Two-stage
Fermentation
Pre-growth stage (24 h)
Initial pH 5
Lipase production stage (72 h)
Initial biomass concentration: 8 gwet weight /L
Response variable
Input variables Morphology (−) pellets (+) dispersed Glucose l-Arabinose Sucrose N:C ratio pH Oleic acid Tributirine CaCl2 FeCl3 Olive oil Specific lipase activity
(U/gdry weight)
Level (g/L)a
 (−) 0.5 CaCl2 0 0 5.0 0.4 5.0 0 0 0 0 10.0
 (+) 1.0 FeCl3 10.0 10.0 10.0 0.6 7.0 1.0 1.0 0.05 0.1 20.0
Trial number
 1 + + + + + + 33.92 ± 1.53
 2 + + + + + + 17.32 ± 3.72
 3 + + + + + + 17.88 ± 3.22
 4 + + + + + + 11.84 ± 1.94
 5 + + + + + + 15.33 ± 0.04
 6 + + + + + + 7.61 ± 0.28
 7 3.12 ± 0.07
 8 + + + + + + 17.19 ± 3.68
 9 + + + + + + 24.47 ± 6.66
 10 + + + + + 11.41 ± 1.51
 11 + + + + + + 20.84 ± 5.88
 12 + + + + + + 4.75 ± 1.81
Effect 3.531 2.747 4.198 9.923 2.232 6.643 − 2.053 − 1.625 − 7.390 1.530 3.978 R2 = 92.73%
p-value 0.022 0.062 0.009 < 0.001 0.121 < 0.001 0.151 0.248 < 0.001 0.275 0.003 R2 adj = 86.07%
  1. Letters in bold show the experimental procedure, variables and statistical analysis corresponding to the pre-growth stage
  2. aConcentration of medium components