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Table 2 Plackett–Burman design: experimental procedure, variables and statistical analysis

From: Orchestration an extracellular lipase production from Aspergillus niger MYA 135: biomass morphology and fungal physiology

Two-stage

Fermentation

Pre-growth stage (24 h)

Initial pH 5

Lipase production stage (72 h)

Initial biomass concentration: 8 gwet weight /L

Response variable

Input variables

Morphology (−) pellets (+) dispersed

Glucose

l-Arabinose

Sucrose

N:C ratio

pH

Oleic acid

Tributirine

CaCl2

FeCl3

Olive oil

Specific lipase activity

(U/gdry weight)

Level (g/L)a

 (−)

0.5 CaCl2

0

0

5.0

0.4

5.0

0

0

0

0

10.0

 (+)

1.0 FeCl3

10.0

10.0

10.0

0.6

7.0

1.0

1.0

0.05

0.1

20.0

Trial number

 1

+

+

+

+

+

+

33.92 ± 1.53

 2

+

+

+

+

+

+

17.32 ± 3.72

 3

+

+

+

+

+

+

17.88 ± 3.22

 4

+

+

+

+

+

+

11.84 ± 1.94

 5

+

+

+

+

+

+

15.33 ± 0.04

 6

+

+

+

+

+

+

7.61 ± 0.28

 7

3.12 ± 0.07

 8

+

+

+

+

+

+

17.19 ± 3.68

 9

+

+

+

+

+

+

24.47 ± 6.66

 10

+

+

+

+

+

11.41 ± 1.51

 11

+

+

+

+

+

+

20.84 ± 5.88

 12

+

+

+

+

+

+

4.75 ± 1.81

Effect

3.531

2.747

4.198

9.923

2.232

6.643

− 2.053

− 1.625

− 7.390

1.530

3.978

R2 = 92.73%

p-value

0.022

0.062

0.009

< 0.001

0.121

< 0.001

0.151

0.248

< 0.001

0.275

0.003

R2 adj = 86.07%

  1. Letters in bold show the experimental procedure, variables and statistical analysis corresponding to the pre-growth stage
  2. aConcentration of medium components