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Table 2 Comparison of transformants and mutation frequency obtained using different sgRNAs targeting fcy1 or pyrG

From: Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus

Plasmid with sgRNA

Hygromycin-resistant strains

5-FC- or 5-FOA-resistant strains

fcy1 or pyrG mutants by PCRa

Rep 1

Rep 2

Rep 3

Rep 1

Rep 2

Rep 3

Rep 1

Rep 2

Rep 3

Empty vector

2

4

8

0b

0b

0b

–

–

–

fcy1 sgRNA No. 1

5

11

18

1 (20%)c

0

0

1/1

–

–

fcy1 sgRNA No. 2

28

23

34

22 (78.6%)c

15 (65.2%)c

30 (88.2%)c

5/5

4/5

4/5

pyrG sgRNA No. 1

17

19

–

8 (47.1%)c

18 (94.7%)c

–

7/8

10/10

–

pyrG sgRNA No. 2

7

7

–

2 (28.6%)c

2 (28.6%)c

–

2/2

2/2

–

  1. aThe number of mutants which can be identified by difference size, or no amplified, bands compared to wild-type control were observed in the genomic PCR experiment (Additional file 1: Fig. S2A and B)
  2. bThe number of 5-FC- or 5-FOA-resistant strains
  3. cPercentage of 5-FC- or 5-FOA-resistant strains from the total number of hygromycin-resistant strains
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