Fig. 2

Identification of fcy1 and pyrG mutations in some strains. a A schematic diagram of the fcy1 and pyrG loci in the PC9 host strain with sgRNA recognition sites. The dash lines indicate the region amplified by genomic PCR. Black arrows display the primer pairs used for the PCR experiments in Additional file 1: Fig. S2A and B. b DNA sequencing to identify small indel mutations in fcy1 or pyrG mutants. For highlights in the nucleotide sequence: yellow shades indicate sgRNA, green shades indicate a protospacer adjacent motif (PAM) sequence, and dash lines indicate deletion