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Table 3 The deletion of kcs1 and arg82 increased the glucose uptake rate and improved the SAM production in yeast

From: Increasing glycolysis by deletion of kcs1 and arg82 improved S-adenosyl-l-methionine production in Saccharomyces cerevisiae

 

2842

Yarg82â–³

Yipk1â–³

Ykcs1â–³

Yarg82â–³kcs1â–³

SAM (g L− 1)

0.66 ± 0.06

0.87 ± 0.05***

0.65 ± 0.03

1.21 ± 0.11***

0.84 ± 0.09***

DCW (g L− 1)

7.85 ± 0.59

6.78 ± 0.19***

6.63 ± 0.22***

6.54 ± 0.16***

6.23 ± 0.12***

SAM yield to DCW (mg  g− 1)

84.1

128.3***

98.0***

185.0***

134.8***

SAM yield to carbon source (mg g− 1)

13.2

17.4***

13.0

24.2***

16.8***

  1. The symbol *** denotes significant diferences between the recombinant strain and the wild strain, with p < 0.001