AMB Express

Table 1 Concentration (mg/L) of the major media components during fermentation of 1.0 wt% mechanocatalytic oligosaccharides in LB media by ethanologenic E. coli KO11 + lgk at 37 °C, 200 rpm

From: Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory

Sugars	LB media	+ 1.0 wt% mechanocatalytic sugars
Sugars	LB media	Initial	24 h	48 h	72 h
Monomers
Glucose	n/a	270	n.d.	n.d.	n.d.
Xylose	n/a	550	n.d.	n.d.	n.d.
Levoglucosan	n/a	16	33	10	7
Malto-oligomers
-ose	n.d.	n.d.	n.d.	n.d.	n.d.
-triose	110	410	490	370	98
-tetraose	120	190	240	170	110
-pentaose	130	300	220	130	130
-hexaose	110	690	570	560	570
-heptaose	140	110	130	160	140
Total	610	1700	1650	1390	1048
Cello-oligomers
-biose	n.d.	n.d.	n.d.	n.d.	n.d.
-triose	98	170	630	500	700
-tetraose	160	320	460	400	500
-pentaose	160	120	130	120	130
-hexaose	170	230	170	170	160
-heptaose	180	120	160	170	160
Total	768	960	1550	1360	1650
Total, all sugars	1400	3500	3200	2800	2700
Products
Dry cell mass	n/a	0.016	1.15 ± 0.03	1.35 ± 0.03	1.38 ± 0.03
Ethanol	n/a	0	2900 ± 200	2600 ± 100	2150 ± 40

Malto- and cello-oligomers are expected to represent the orientation of the glycosidic bond, but not necessarily the linkage. Glucose, xylose and levoglucosan were measured by IEC, other sugars by HPLC. Ethanol was measured by GC and dry biomass content was estimated from OD₅₅₀ values
n/a not determined, n.d. none detected

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