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AMB Express

Table 1 Comparison between the “in vitro” biological activity of CHO-derived (Infuse) and of E. coli-derived (GenScript) hBMP-2 preparations

From: Human bone morphogenetic protein-2 (hBMP-2) characterization by physical–chemical, immunological and biological assays

Assay #

E. coli-derived hBMP-2

CHO-derived hBMP-2

Relative potency of CHO-derived hBMP-2 with basis on:

Slopea (A405/µg/mL)

ED50 (µg/mL)

Slopea (A405/µg/mL)

ED50 (µg/mL)

Slope (ratio)

ED50 (ratio)

1

0.637

2.50

3.081

0.40

4.8

6.2

2

0.495

1.15

3.147

0.48

6.3

2.4

3

0.543

1.18

2.056

0.40

3.8

3.0

4

0.565

1.47

2.403

0.50

4.2

2.9

5

0.704

1.23

2.746

0.47

3.9

2.6

Mean ± SD

0.589 ± 0.082

1.51 ± 0.57

2.687 ± 0.46

0.45 ± 0.047

4.60 ± 1.03

3.42 ± 1.57

CV (%)

14

38

17

10

22

46

  1. aSlope of the dose–response curve: YA405 = a Xµg/mL + b. In the case of CHO-derived hBMP-2 the equations were calculated with basis on the initial linear region of the dose–response curve
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