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Table 1 Comparison between the “in vitro” biological activity of CHO-derived (Infuse) and of E. coli-derived (GenScript) hBMP-2 preparations

From: Human bone morphogenetic protein-2 (hBMP-2) characterization by physical–chemical, immunological and biological assays

Assay #E. coli-derived hBMP-2CHO-derived hBMP-2Relative potency of CHO-derived hBMP-2 with basis on:
Slopea (A405/µg/mL)ED50 (µg/mL)Slopea (A405/µg/mL)ED50 (µg/mL)Slope (ratio)ED50 (ratio)
10.6372.503.0810.404.86.2
20.4951.153.1470.486.32.4
30.5431.182.0560.403.83.0
40.5651.472.4030.504.22.9
50.7041.232.7460.473.92.6
Mean ± SD0.589 ± 0.0821.51 ± 0.572.687 ± 0.460.45 ± 0.0474.60 ± 1.033.42 ± 1.57
CV (%)143817102246
  1. aSlope of the dose–response curve: YA405 = a Xµg/mL + b. In the case of CHO-derived hBMP-2 the equations were calculated with basis on the initial linear region of the dose–response curve