Fig. 5
Duplication of the 200 kb to 400 kb chromosomal region in Chr15. Region Chr15-L1 to Chr15-L5 of Chr15 were selected. All the duplicating modules were marked with CgHIS3 along with CEN4 and CgLEU2. Fig. 5a represents PFGE analysis of wild type SJY30 and transformants obtained from the duplication experiments for the Chr15-L1, Chr15-L2, Chr15-L3, Chr15-L4 and Chr15-L5 regions by CRISPR-PCDup. Fig. 5b shows the results of Southern blot analysis after PFGE for the detection of the newly generated 400 kb, 350 kb, 300 kb, 250 kb and 200 kb chromosomes, respectively