Fig. 4

Colony PCR analysis of Chr3-1 and Chr8-1 as well as Chr3-1 and Chr14-4 sequentially duplicated transformants. In the colony PCR, each lane represents independent transformants. Fig. 4a, b represents the sequential duplication of the Chr3-1 and Chr8-1 regions, respectively. In Fig. 4a, primers SJP 555 and SJP 411 were used to amplify the 1.5 kb band from the duplicated Chr8-1 region and in Fig. 4b, primers SJP 510 and SJP 119 were used to amplify the 1 kb band from the duplicated Chr3-1 region. Fig. 4c , d represents the sequential duplication of the Chr3-1 and Chr14-4 regions, respectively. In Fig. 4c, primers SJP 550 and SJP 668 were used to amplify the 1.4 kb band from the duplicated Chr14-4 region and in Fig. 4d, primers SJP 510 and SJP 119 were used to amplify the 1 kb band from the duplicated Chr3-1 region. In all PCR analysis the 0.67kb CNE1 gene on Chromosome 1 was also amplified as an internal control by a common set of primers SJP 121 and SJP 242