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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: CRISPR-PCDup: a novel approach for simultaneous segmental chromosomal duplication in Saccharomyces cerevisiae

Fig. 1

Outline of the CRISPR-PCDup method. gRNA expressing plasmid and duplicating modules are introduced into the SJY30 strain, which harbors a Cas9-expressing plasmid. In transformed cells, CRISPR/Cas9 mediated double-strand breaks (DSBs) are induced near the target site. Chromosome duplication is then facilitated by PCDup. This new technology is named CRISPR-PCDup

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