Fig. 1

The construction map of the pBPxhE-S-Bm. The full-length S gene of PEDV were cloned into the pBPxhE plasmid and formed the pBPxhE-S-Bm in attempt to produce S protein anchored BmNPV. The original transmembrane domain (TM) and the cytoplasmic domain (CTD) of PEDV were retained for membrane anchoring. The expression of target gene was triggered by polyhedrin (polh) promoter which was followed by the signal peptide of GP64 and 6× His tag. In additionally, both constructed had an EGFP reporter that driven by the heat shock protein (Hsp) promoter