Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditions

AMB Express

Table 1 Comparison of two feeding strategies

Strategy	Time	Rate (mL min⁻¹)	Volume (mL)	Ingredient
DO-stat feeding	DO rebound period	0.1	100	Sucrose
Constant-rate feeding	Late logarithmic growth	0.1	100	Sucrose