Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
Escherichia coli | ||
TOP10 | mcrA, Δ(mrr-hsdRMS-mcrBC), Phi80lacZ(del)M15, ΔlacX74, deoR, recA1, araD139, Δ(ara-leu)7697, galU, galK, rpsL(SmR), endA1, nupG, strain used for general cloning procedures | Invitrogen |
C43(DE3) | F– ompT gal dcm hsdSB(rB- mB-)(DE3), strain used for protein expression | Miroux and Walker (1996) |
Plasmids | ||
pSenSox | AmpR; pBtac-Lbadh derivative containing the soxRS-based NADPH biosensor and the LbadhWT gene under transcriptional control of the tac promoter | Siedler et al. (2014) |
pSenNeg | AmpR; pSenSox derivative with an incomplete LbadhWT gene preventing synthesis of an active LbAdhWT | Siedler et al. (2014) |
pSenSox-LbadhK71E | AmpR; pSenSox derivative with the LbadhK71E gene under control of the tac-promoter | This study |
pASK-IBA5plus-LbadhWT | AmpR; pASK-IBA5plus derivative for production of LbAdhWT with N-terminal Strep-tag II under control of the tet-promoter/operator | Prof. W. Kroutil, Department of Chemistry, University of Graz, Austria |
pASK-IBA5plus-LbadhK71E | AmpR; pASK-IBA5plus derivative carrying the gene construct for the purification of the mutant LbAdhK71E protein with an N-terminal Strep-Tactin affinity tag (Strep-tag II) under transcriptional control of the tet-promoter/operator | This study |