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Table 1 Bacterial strains and plasmids used in this study

From: NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Strain or plasmid Relevant characteristics Source or reference
Escherichia coli
 TOP10 mcrA, Δ(mrr-hsdRMS-mcrBC), Phi80lacZ(del)M15, ΔlacX74, deoR, recA1, araD139, Δ(ara-leu)7697, galU, galK, rpsL(SmR), endA1, nupG, strain used for general cloning procedures Invitrogen
 C43(DE3) F– ompT gal dcm hsdSB(rB- mB-)(DE3), strain used for protein expression Miroux and Walker (1996)
 pSenSox AmpR; pBtac-Lbadh derivative containing the soxRS-based NADPH biosensor and the LbadhWT gene under transcriptional control of the tac promoter Siedler et al. (2014)
 pSenNeg AmpR; pSenSox derivative with an incomplete LbadhWT gene preventing synthesis of an active LbAdhWT Siedler et al. (2014)
 pSenSox-LbadhK71E AmpR; pSenSox derivative with the LbadhK71E gene under control of the tac-promoter This study
 pASK-IBA5plus-LbadhWT AmpR; pASK-IBA5plus derivative for production of LbAdhWT with N-terminal Strep-tag II under control of the tet-promoter/operator Prof. W. Kroutil, Department of Chemistry, University of Graz, Austria
 pASK-IBA5plus-LbadhK71E AmpR; pASK-IBA5plus derivative carrying the gene construct for the purification of the mutant LbAdhK71E protein with an N-terminal Strep-Tactin affinity tag (Strep-tag II) under transcriptional control of the tet-promoter/operator This study