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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Fig. 1

FACS analysis of the LbADH mutant library. Comparison of the fluorescence distribution of the E. coli TOP10/pSenSox-LbadhLibrary culture before (blue) and after three positive enrichment steps followed by one negative and another positive enrichment step (green). E. coli TOP10/pSenSox cells (orange) were used as positive control and E. coli TOP10/pSenNeg cells (gray) as negative control. Before FACS analysis, cells of the different cultures were incubated for 2.5 h with the substrate 2,5-hexanedione. Prior to sorting, 2.5 × 105 cells of each culture were analyzed. The plots were generated with the BD DIVA 6.1.3 software. a Histogram of the four E. coli cultures described above. b Dot plots of the four cultures described above displaying the eYFP fluorescence signal against the forward scatter height (FSC-H) reflecting the size of the cells

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