Fig. 4

Polymerization of caseinate by SmTG extracted from BY4741-TG and GCI-TG strains. Each sample volume loaded was 20 µL (a) and 5 µL (b). a Lane 1, protein molecular size marker; lane 2, caseinate control; lane 3, polymerized caseins by 12×SmTG extracted from the BY4741-TG strain; lane 4, polymerized caseins by 12×SmTG extracted from the GCI-TG strain; lane 5, BY4741-TG extract; lane 6, GCI-TG extract. b Expanded region of the SDS-PAGE in (a boxed region). Ratios of cross-linked α-casein were calculated from the intensity of each α-casein band and represented as relative values (%). c The concentration of proteins whose molecular weights were more than 100 kDa, were detected after polymerization of caseinate by 12×SmTG extracted from the BY4741-TG and GCI-TG strain