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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: High repetitive arginine in the anterior of PCV3 capsid protein is a severe obstacle for its expression in E. coli

Fig. 1

Expression and identification of recombinant PCV3 capsid proteins expressed in E. coli. a The SDS-PAGE analysis of the recombined capsid protein of PCV3. M, protein marker; 1, control bacteria with pET-28a; 2, the bacteria with pET-3CAP; 3, the bacteria with pET-Δ3CAP; 4, the bacteria with pET-3CAP-O; 5, the bacteria with pET-Δ3CAP-O. b Western blots analysis of the expressed recombined proteins using the anti-His antibody. The blot corresponds to lanes 1–5 from (a). The arrows represent the location of the recombined ΔCAP (25 kDa), respectively. The expression of the recombined CAP protein did not be detected

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