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AMB Express
Fig. 5 | AMB Express

Fig. 5

From: Screening and selection strategy for the establishment of biosimilar to trastuzumab-expressing CHO-K1 cell lines

Fig. 5

Correlation between intracellular antibody polypeptides content and concentration of IgG in cell culture supernatants. Cell mini-pools were seeded in 24 wells plate in CP-CHO-CB5-FBS medium. After 10 days concentration of IgG in cell culture supernatants was measured by ELISA. Intracellular HC and LC polypeptides of ethanol fixed cells were assessed by flow cytometry. MFI/MFICHO-K1: mean fluorescence intensity of trastuzumab-expressing cells compared to the mean fluorescence intensity of CHO-K1 wild type cells ratio. The correlation between variables was computed using a parametric test based on Pearson correlation

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