Table 1 Primer sequences of Bax, Caspase-3, and GAPDH
Gene | Sequence | Primer sequence | Annealing | Product size (bp) |
|---|---|---|---|---|
Bax | F R | TTCCGAGTGGCAGCTGAGATGTTT TGCTGGCAAAGTAGAAGAGGGCAA | 54 °C × 30 s | 108 |
Caspase-3 | F R | ACTGGACTGTGGCATTGAGA GCACAAAGCGACTGGATGAA | 54 °C × 30 s | 70 |
GAPDH | F R | ATCTGACATGCTGCCTGGAG AAGGTTGGAAGATGGGAGTTGC | 60 °C × 25 s | 88 |