Table 1 Sixteen genome libraries generated from combination of eight adaptors sequences randomly digested by MnlI restriction enzyme
No | Genome library names | Adaptor combinationa | 5′Adaptor name | 5′ Adaptor sequence | 3′ Adaptor name | 3′ Adaptor sequence |
|---|---|---|---|---|---|---|
1 | AA | 5AA-3AA | 5AA | 5′ ATGGATAGGTCGGTGA 3′ (R1S) 3′A-TACCTATCCAGCCACT 5′ (F1A) | 3AA | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′A-CTGCGTGGAACTCCG 5′ (F2A) |
2 | AT | 5AA-3AT | 3AT | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′T-CTGCGTGGAACTCCG 5′ (F2T) | ||
3 | AG | 5AA-3AG | 3AG | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′G-CTGCGTGGAACTCCG 5′ (F2G) | ||
4 | AC | 5AA-3AC | 3AC | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′C-CTGCGTGGAACTCCG 5′ (F2C) | ||
5 | TA | 5TA-3AA | 5TA | 5′ ATGGATAGGTCGGTGA 3′ (R1S) 3′T-TACCTATCCAGCCACT 5′ (F1T) | 3AA | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′A-CTGCGTGGAACTCCG 5′ (F2A) |
6 | TT | 5TA-3AT | 3AT | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′T-CTGCGTGGAACTCCG 5′ (F2T) | ||
7 | TG | 5TA-3AG | 3AG | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′G-CTGCGTGGAACTCCG 5′ (F2G) | ||
8 | TC | 5TA-3AC | 3AC | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′C-CTGCGTGGAACTCCG 5′ (F2C) | ||
9 | GA | 5GA-3AA | 5GA | 5′ ATGGATAGGTCGGTGA 3′ (R1S) 3′G-TACCTATCCAGCCACT 5′ (F1G) | 3AA | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′A-CTGCGTGGAACTCCG 5′ (F2A) |
10 | GT | 5GA-3AT | 3AT | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′T-CTGCGTGGAACTCCG 5′ (F2T) | ||
11 | GG | 5GA-3AG | 3AG | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′G-CTGCGTGGAACTCCG 5′ (F2G) | ||
12 | GC | 5GA-3AC | 3AC | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′C-CTGCGTGGAACTCCG 5′ (F2C) | ||
13 | CA | 5CA-3AA | 5CA | 5′ ATGGATAGGTCGGTGA 3′ (R1S) 3′C-TACCTATCCAGCCACT 5′ (F1C) | 3AA | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′A-CTGCGTGGAACTCCG 5′ (F2A) |
14 | CT | 5CA-3AT | 3AT | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′T-CTGCGTGGAACTCCG 5′ (F2T) | ||
15 | CG | 5CA-3AG | 3AG | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′G-CTGCGTGGAACTCCG 5′ (F2G) | ||
16 | CC | 5CA-3AC | 3AC | 5′ GACGCACCTTGAGGC 3′ (R2S) 3′C-CTGCGTGGAACTCCG 5′ (F2C) |