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Table 1 Sixteen genome libraries generated from combination of eight adaptors sequences randomly digested by MnlI restriction enzyme

From: Identification of stress-responsive transcription factors with protein-bound Escherichia coli genomic DNA libraries

No Genome library names Adaptor combinationa 5′Adaptor name 5′ Adaptor sequence 3′ Adaptor name 3′ Adaptor sequence
1 AA 5AA-3AA 5AA 5′ ATGGATAGGTCGGTGA 3′ (R1S)
3′A-TACCTATCCAGCCACT 5′ (F1A)
3AA 5′ GACGCACCTTGAGGC 3′ (R2S)
3′A-CTGCGTGGAACTCCG 5′ (F2A)
2 AT 5AA-3AT 3AT 5′ GACGCACCTTGAGGC 3′ (R2S)
3′T-CTGCGTGGAACTCCG 5′ (F2T)
3 AG 5AA-3AG 3AG 5′ GACGCACCTTGAGGC 3′ (R2S)
3′G-CTGCGTGGAACTCCG 5′ (F2G)
4 AC 5AA-3AC 3AC 5′ GACGCACCTTGAGGC 3′ (R2S)
3′C-CTGCGTGGAACTCCG 5′ (F2C)
5 TA 5TA-3AA 5TA 5′ ATGGATAGGTCGGTGA 3′ (R1S)
3′T-TACCTATCCAGCCACT 5′ (F1T)
3AA 5′ GACGCACCTTGAGGC 3′ (R2S)
3′A-CTGCGTGGAACTCCG 5′ (F2A)
6 TT 5TA-3AT 3AT 5′ GACGCACCTTGAGGC 3′ (R2S)
3′T-CTGCGTGGAACTCCG 5′ (F2T)
7 TG 5TA-3AG 3AG 5′ GACGCACCTTGAGGC 3′ (R2S)
3′G-CTGCGTGGAACTCCG 5′ (F2G)
8 TC 5TA-3AC 3AC 5′ GACGCACCTTGAGGC 3′ (R2S)
3′C-CTGCGTGGAACTCCG 5′ (F2C)
9 GA 5GA-3AA 5GA 5′ ATGGATAGGTCGGTGA 3′ (R1S)
3′G-TACCTATCCAGCCACT 5′ (F1G)
3AA 5′ GACGCACCTTGAGGC 3′ (R2S)
3′A-CTGCGTGGAACTCCG 5′ (F2A)
10 GT 5GA-3AT 3AT 5′ GACGCACCTTGAGGC 3′ (R2S)
3′T-CTGCGTGGAACTCCG 5′ (F2T)
11 GG 5GA-3AG 3AG 5′ GACGCACCTTGAGGC 3′ (R2S)
3′G-CTGCGTGGAACTCCG 5′ (F2G)
12 GC 5GA-3AC 3AC 5′ GACGCACCTTGAGGC 3′ (R2S)
3′C-CTGCGTGGAACTCCG 5′ (F2C)
13 CA 5CA-3AA 5CA 5′ ATGGATAGGTCGGTGA 3′ (R1S)
3′C-TACCTATCCAGCCACT 5′ (F1C)
3AA 5′ GACGCACCTTGAGGC 3′ (R2S)
3′A-CTGCGTGGAACTCCG 5′ (F2A)
14 CT 5CA-3AT 3AT 5′ GACGCACCTTGAGGC 3′ (R2S)
3′T-CTGCGTGGAACTCCG 5′ (F2T)
15 CG 5CA-3AG 3AG 5′ GACGCACCTTGAGGC 3′ (R2S)
3′G-CTGCGTGGAACTCCG 5′ (F2G)
16 CC 5CA-3AC 3AC 5′ GACGCACCTTGAGGC 3′ (R2S)
3′C-CTGCGTGGAACTCCG 5′ (F2C)
  1. a Each adaptor was abbreviated with 5AA (5′ adaptor A), 5AT (5′ adaptor T), 5AG (3′ adaptor G), etc. 5′ adaptors are annealed with shared sequence R1S while 3′ adaptors were annealed with sharded sequence R2S. Each 5′ adaptor was combined with each 3′ adaptor to create a total of 16 genome libraries