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Table 3 Validation parameters for indirect ELISA using positive and negative control serum samples from goats and sheep. rSodC protein and secreted C. pseudotuberculosis antigens were used as test antigens

From: rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay

Parameter Goat Sheep
rSodC Secreted antigen rSodC Secreted antigen
Positive control 50 50 45 45
Negative control 50 50 45 45
Cut-off 0.275 0.274 0.332 0.510
Sensitivity (%) 96 100 95 98
Specificity (%) 94 96 98 93
Accuracy (%) 97.6 99.8 98.9 99.5
Positive predictive value (%) 100 100 100 100
Negative predictive value (%) 93.7 99.5 97.1 98.7