Table 3 Validation parameters for indirect ELISA using positive and negative control serum samples from goats and sheep. rSodC protein and secreted C. pseudotuberculosis antigens were used as test antigens
Parameter | Goat | Sheep | ||
|---|---|---|---|---|
rSodC | Secreted antigen | rSodC | Secreted antigen | |
Positive control | 50 | 50 | 45 | 45 |
Negative control | 50 | 50 | 45 | 45 |
Cut-off | 0.275 | 0.274 | 0.332 | 0.510 |
Sensitivity (%) | 96 | 100 | 95 | 98 |
Specificity (%) | 94 | 96 | 98 | 93 |
Accuracy (%) | 97.6 | 99.8 | 98.9 | 99.5 |
Positive predictive value (%) | 100 | 100 | 100 | 100 |
Negative predictive value (%) | 93.7 | 99.5 | 97.1 | 98.7 |