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Fig. 3 | AMB Express

Fig. 3

From: Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites

Fig. 3

Decitabine scale-up bioprocess. A constant flow of reaction mixture of 7, 15, 20 and 30 mL/min was evaluated in packed bed design, while an air flow of 0.1, 0.2, 0.4 and 0.6 L/h was evaluated in airlift model. Biosynthesis was carried out in 15 mL using 0.1 g/mL of biocatalyst, 30 mM dThd, and 10 mM 5-azaCyt in Tris–HCl buffer (20 mM, pH 7) at 30 °C. The values correspond to 2 h of reaction

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