Fig. 1

Heat shock stimulates mitotic recombination in the yeast strain JSC25-1. a A reciprocal crossover event on the right arm of chromosome IV would produce white/red-sectored JSC25-1 colonies. Alternatively, break-induced replication (nonreciprocal crossover) would produce white/pink- or red/pink-sectored colonies. b Cell viability of the JSC25-1 yeast strain after heat shock exposure (52 °C) for 2–4 min. c Reciprocal crossover frequency determined by calculating the red/white-sectored colonies formed on YPD plates after heat shock. “*” indicates significant difference at the level of 0.01 using t-test