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Table 1 E. coli strains and plasmids used in the study

From: Efficient ammonia production from food by-products by engineered Escherichia coli

Strains/plasmids Description Source
E. coli strains
 DH10B F- mcrAΔ(mrr-hsdRMS-mcrBC) φ80d lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(araleu)7697 galU galK- rpsL nupG Thermo fisher
 DH10B (pKD46) DH10B harboring pKD46 Mikami et al. (2017)
 ∆glnA DH10B, ΔglnA::FRT-kanR-FRT Mikami et al. (2017)
 ∆ptsG DH10B, ΔptsG::FRT-kanR-FRT This study
 ∆ptsG-kan DH10B, ΔptsG::FRT This study
 ∆ptsG∆glnA DH10B, ΔptsG::FRT ΔglnA::FRT-kanR-FRT This study
Plasmids
 pKD46 Red recombinase expression plasmid Datsenko et al. (2000)
 pKD13 KanR template plasmid Datsenko et al. (2000)
 pCP20 Temperature-sensitive replication and thermal induction of FLP synthesis Cherepanov and Wackernagel (1995)