Table 1 E. coli strains and plasmids used in the study
From: Efficient ammonia production from food by-products by engineered Escherichia coli
Strains/plasmids | Description | Source |
|---|---|---|
E. coli strains | ||
DH10B | F- mcrAΔ(mrr-hsdRMS-mcrBC) φ80d lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(araleu)7697 galU galK- rpsL nupG | Thermo fisher |
DH10B (pKD46) | DH10B harboring pKD46 | Mikami et al. (2017) |
∆glnA | DH10B, ΔglnA::FRT-kanR-FRT | Mikami et al. (2017) |
∆ptsG | DH10B, ΔptsG::FRT-kanR-FRT | This study |
∆ptsG-kan | DH10B, ΔptsG::FRT | This study |
∆ptsG∆glnA | DH10B, ΔptsG::FRT ΔglnA::FRT-kanR-FRT | This study |
Plasmids | ||
pKD46 | Red recombinase expression plasmid | Datsenko et al. (2000) |
pKD13 | KanR template plasmid | Datsenko et al. (2000) |
pCP20 | Temperature-sensitive replication and thermal induction of FLP synthesis | Cherepanov and Wackernagel (1995) |