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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Improving the production of AHL lactonase AiiO-AIO6 from Ochrobactrum sp. M231 in intracellular protease-deficient Bacillus subtilis

Fig. 4

Secretion of AiiO-AIO6 by intracellular protease-deficient strains. a SDS-PAGE (up) and Western blotting (down) of AiiO-AIO6 secreted in culture medium derived from wild-type (WT) strain 1A751 or intracellular protease-deficient mutants after different time points cultivation at 37 °C. M, protein marker; CK, pWB980/BS1A751, wild-type (WT), pWB980-AIO6BS/BS1A751; ∆ymfH, ∆yrrN, ∆ywpE, ∆tepA: intracellular protease-deficient mutants harboring the expression vector pWB980-AIO6BS. b Secreted AiiO-AIO6 activity present in wild-type or intracellular protease-deficient mutant culture medium. p values were calculated using unpaired two-tailed T-tests. An asterisk indicates a statistically significant difference between two activity values (p < 0.05)

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