Table 1 Bacterial strains, plasmids, and primers used in this study
Bacterial strains or plasmids | Description | Reference |
|---|---|---|
Bacterial strains | ||
Escherichia coli DH5α | F−, φ 80dlacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rK−, mK+), phoA, supE44, λ−, thi-1, gyrA96, relA1 | Hanahan (1983) |
Bacillus subtilis 168 | trpC2 | Anagnostopoulos and Crawford (1961) |
Lactobacillus rhamnosus GG | ATCC 53103 | Purchased from ATCC |
Listeria monocytogenes | ATCC19115 | Purchased from ATCC |
Plasmids | ||
pUB19 | E. coli-B. subtilis shuttle vector, Apr, Kmr | Kang et al. (2019) |
pUB19-cotG-p75 | Spore display of p75 using the CotG anchor | This study |
Primers | ||
CotG-F | 5′-CTTCGACGCGTCAGCTGGCACC-3′ | This study |
CotG-R | 5′-GTCCCTGTCGAGCTTCCTCCTCCTCCTTTGTATTTCTTTTTGACTACCCAGCAATTGCCGT-3′ | This study |
P75-F | 5′-AGAAATACAAAGGAGGAGGAGGAAGCTCGACAGGGACGGTCAGTTACAAATCCGG-3′ | This study |
P75-R | 5′-AAGGAAAAAGCGGCCGCTTATAGTGACGGGCGAACCGCAAAGTCAGG-3′ | This study |