Skip to main content

Table 1 Bacterial strains, plasmids, and primers used in this study

From: Surface display of p75, a Lactobacillus rhamnosus GG derived protein, on Bacillus subtilis spores and its antibacterial activity against Listeria monocytogenes

Bacterial strains or plasmids Description Reference
Bacterial strains
 Escherichia coli DH5α F, φ 80dlacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rK, mK+), phoA, supE44, λ, thi-1, gyrA96, relA1 Hanahan (1983)
 Bacillus subtilis 168 trpC2 Anagnostopoulos and Crawford (1961)
 Lactobacillus rhamnosus GG ATCC 53103 Purchased from ATCC
 Listeria monocytogenes ATCC19115 Purchased from ATCC
Plasmids
 pUB19 E. coli-B. subtilis shuttle vector, Apr, Kmr Kang et al. (2019)
 pUB19-cotG-p75 Spore display of p75 using the CotG anchor This study
Primers
 CotG-F 5′-CTTCGACGCGTCAGCTGGCACC-3′ This study
 CotG-R 5′-GTCCCTGTCGAGCTTCCTCCTCCTCCTTTGTATTTCTTTTTGACTACCCAGCAATTGCCGT-3′ This study
 P75-F 5′-AGAAATACAAAGGAGGAGGAGGAAGCTCGACAGGGACGGTCAGTTACAAATCCGG-3′ This study
 P75-R 5′-AAGGAAAAAGCGGCCGCTTATAGTGACGGGCGAACCGCAAAGTCAGG-3′ This study
  1. a ATCC, American type culture collection (Manassas, VA, USA)
  2. b The bold letters indicate the restriction sites
  3. c The underlined letters indicate a flexible linker (GGGGS) at the C-terminal of the cotG structural gene