Table 3 Oligonucleotide primers designed for development of species-specific PCR and Duplex-qPCR assays.
Pathogen | Primers | Sequence (5ʹ– 3ʹ) | Length | Product size | Annealing temp. ( °C) | Melting curve peak ( °C) |
|---|---|---|---|---|---|---|
P. cubensis | PcK F | GCTGGTTGATTACTGCTTGGCG | 22 | 705 bp | 58 | 88.05 |
PcK R | CCGAAGCCACACAACACATAG | 21 | ||||
P. xanthii | PxK F | CCCGTGTGAACTCTTATCTG | 20 | 290 bp | 85.83 | |
PxK R | GAGGGGTGTTCTGACGCTCG | 20 |