The expression of NESTIN in three different culture conditions. a, b The flow cytometry analysis of NESTIN in UC-MSCs. c The percentage of NESTIN+ cells in three culture conditions. d The expression level of NESTIN in three culture conditions. e–h The percentage of NESTIN+ cells and expression level of NESTIN after changing the medium. **p < 0.01, * p < 0.05, Student’s t-test. For all experiments, n = 3 healthy donors/group, and data shown are mean ± SD.