The characterization of UC-MSCs in three different culture conditions. a The UC-MSCs at the 10th passage in three culture conditions were positive for CD29, CD90, CD105, and CD73, and were negative for CD45, CD34, CD79a, HLA-DR, and CD14. b The flow cytometry statistics of stem cell markers in three culture conditions. c The UC-MSCs at the 10th passage in three culture conditions were stained with Alcian blue, oil red O, and Alizarin Red S, respectively. For all experiments, n = 3 healthy donors/group, and data shown are mean ± SD.