Table 2 The extraction yield of anti-G17-Gly scFv (mg/L) for a central composite design comprising 4 variables
Run | Tris (mM) | EDTA (mM) | pH | Time (min) | Anti-G17-Gly scFv (mg/L) | ||||
|---|---|---|---|---|---|---|---|---|---|
1 | 50 | − 1 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 1.47 |
2 | 50 | − 1 | 2 | + 1 | 9 | + 1 | 60 | + 1 | 1 |
3 | 200 | + 1 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 0.34 |
4 | 50 | − 1 | 0.1 | − 1 | 7.2 | − 1 | 60 | + 1 | 3.0 |
5 | 125 | 0 | 2 | + 1 | 8.1 | 0 | 37.5 | 0 | 0.31 |
6 | 50 | − 1 | 0.1 | − 1 | 9 | + 1 | 60 | + 1 | 3.16 |
7 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 0.39 |
8 | 50 | − 1 | 2 | + 1 | 7.2 | − 1 | 60 | + 1 | 3.3 |
9 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 0.415 |
10 | 200 | + 1 | 2 | + 1 | 9 | + 1 | 15 | − 1 | 0.58 |
11 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 0.414 |
12 | 125 | 0 | 1.05 | 0 | 7.2 | − 1 | 37.5 | 0 | 2 |
13 | 200 | + 1 | 2 | + 1 | 7.2 | − 1 | 60 | + 1 | 0.64 |
14 | 200 | + 1 | 0.1 | − 1 | 9 | + 1 | 15 | − 1 | 0.18 |
15 | 50 | − 1 | 0.1 | − 1 | 9 | + 1 | 15 | − 1 | 1.65 |
16 | 50 | − 1 | 2 | + 1 | 7.2 | − 1 | 15 | − 1 | 2.17 |
17 | 200 | + 1 | 0.1 | − 1 | 7.2 | − 1 | 15 | − 1 | 0.43 |
18 | 50 | − 1 | 2 | + 1 | 9 | + 1 | 15 | − 1 | 0.77 |
19 | 200 | + 1 | 2 | + 1 | 7.2 | − 1 | 15 | − 1 | 0.9 |
20 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 0.412 |
21 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 0.4 |
22 | 50 | − 1 | 0.1 | − 1 | 7.2 | − 1 | 15 | − 1 | 2.54 |
23 | 125 | 0 | 1.05 | 0 | 9 | + 1 | 37.5 | 0 | 0.3 |
24 | 200 | + 1 | 2 | + 1 | 9 | + 1 | 60 | + 1 | 0.39 |
25 | 200 | + 1 | 0.1 | − 1 | 7.2 | − 1 | 60 | + 1 | 0.3 |
26 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 15 | − 1 | 1 |
27 | 200 | + 1 | 0.1 | − 1 | 9 | + 1 | 60 | + 1 | 0.45 |
28 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 37.5 | 0 | 0.42 |
29 | 125 | 0 | 0.1 | − 1 | 8.1 | 0 | 37.5 | 0 | 0.42 |
30 | 125 | 0 | 1.05 | 0 | 8.1 | 0 | 60 | + 1 | 0.68 |