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Table 1 Experimental deign levels. Values of independent variables and corresponding coded levels were used in a central composite design

From: Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

Symbols Variables Real values of coded levels
− 1 0  + 1
A Tris (mM) 50 125 200
B EDTA (mM) 0.1 1.05 2
C pH 7.2 8.1 9
D Time (min) 15 37.5 60
  1. The central point (0) mentions middle values interval between the − 1 and + 1 of each variable