From: Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies
Symbols
Variables
Real values of coded levels
− 1
0
+ 1
A
Tris (mM)
50
125
200
B
EDTA (mM)
0.1
1.05
2
C
pH
7.2
8.1
9
D
Time (min)
15
37.5
60