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Table 2 Percentage of the average anucleated cell number in rhodomyrtone treated-recombinant Streptococcus suis ParB-GFP

From: Antibacterial mechanism of rhodomyrtone involves the disruption of nucleoid segregation checkpoint in Streptococcus suis

Time (t) in minutePercentage of average anucleated cell number (%)
1% DMSORhodomyrtone (µg/ml)1 µg/ml
Rifampicin
4 µg/ml
Quinolone
10.50.250.1250.062
000000000
1500000000
3000.3 ± 0.200000.7 ± 0.2*0.9 ± 0.2*
4500.3 ± 0.2*00001.1 ± 0.2*2.1 ± 0.2*
6002.3 ± 0.2*1.4 ± 0.4*0.7 ± 0.2*0.1 ± 0.204.1 ± 0.6*6.3 ± 0.2**
12003.4 ± 0.4*1.9 ± 0.2*0.7 ± 0.2*0.1 ± 0.20.1 ± 0.24.8 ± 0.4*6.4 ± 0.2**
24003.5 ± 0.4*2.1 ± 0.2*1.3 ± 0.2*0.7 ± 0.2*0.3 ± 0.25.2 ± 0.4*6.6 ± 0.4*
  1. Cells were exposed to 0.062 to 1 µg/ml rhodomyrtone, 1 µg/ml rifampicin, and 4 µg/ml quinolone. DNA was stained by Hoechst 33342 and visualized. Anucleated cells were determined by absence of Hoechst signal (blue). For each treatment, over 500 cells were counted. The results were shown as mean ± SD of three independent experiments
  2. *The significant changes in anucleated cell numbers between different treatments and durations were compared to 1% DMSO negative control using the Student’s t-test. Significant differences were indicated as * (P-values < 0.05) and ** (P-values < 0.001)