Antibacterial mechanism of rhodomyrtone involves the disruption of nucleoid segregation checkpoint in Streptococcus suis

AMB Express

Table 2 Percentage of the average anucleated cell number in rhodomyrtone treated-recombinant Streptococcus suis ParB-GFP

Time (t) in minute	Percentage of average anucleated cell number (%)
	1% DMSO	Rhodomyrtone (µg/ml)					1 µg/ml Rifampicin	4 µg/ml Quinolone
	1% DMSO	1	0.5	0.25	0.125	0.062	1 µg/ml Rifampicin	4 µg/ml Quinolone
0	0	0	0	0	0	0	0	0
15	0	0	0	0	0	0	0	0
30	0	0.3 ± 0.2	0	0	0	0	0.7 ± 0.2*	0.9 ± 0.2*
45	0	0.3 ± 0.2*	0	0	0	0	1.1 ± 0.2*	2.1 ± 0.2*
60	0	2.3 ± 0.2*	1.4 ± 0.4*	0.7 ± 0.2*	0.1 ± 0.2	0	4.1 ± 0.6*	6.3 ± 0.2**
120	0	3.4 ± 0.4*	1.9 ± 0.2*	0.7 ± 0.2*	0.1 ± 0.2	0.1 ± 0.2	4.8 ± 0.4*	6.4 ± 0.2**
240	0	3.5 ± 0.4*	2.1 ± 0.2*	1.3 ± 0.2*	0.7 ± 0.2*	0.3 ± 0.2	5.2 ± 0.4*	6.6 ± 0.4*

Cells were exposed to 0.062 to 1 µg/ml rhodomyrtone, 1 µg/ml rifampicin, and 4 µg/ml quinolone. DNA was stained by Hoechst 33342 and visualized. Anucleated cells were determined by absence of Hoechst signal (blue). For each treatment, over 500 cells were counted. The results were shown as mean ± SD of three independent experiments
*The significant changes in anucleated cell numbers between different treatments and durations were compared to 1% DMSO negative control using the Student’s t-test. Significant differences were indicated as * (P-values < 0.05) and ** (P-values < 0.001)