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Fig. 2 | AMB Express

Fig. 2

From: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

Fig. 2

Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE (a, c) and Far-western blot analysis (b, d) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

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