Prevalence, the antibiogram and the frequency of virulence genes of the most predominant bacterial pathogens incriminated in calf pneumonia

AMB Express

Table 1 Primer sequences and PCR cycling conditions applied for the detection of virulence genes of E. coli, S. aureus and P. multocida strains isolated from examined calves

Bacterial Species	Target gene	Primer sequence (5′-3′)	Size (bp)	PCR cycling conditions (^°C/s) for 35 cycles			References
Bacterial Species	Target gene	Primer sequence (5′-3′)	Size (bp)	D	A	E	References
E. coli	fimH	TGCAGAACGGATAAGCCGTGG GCAGTCACCTGCCCTCCGGTA	508	94/30	50/40	72/40	Ghanbarpour and Salehi (2010)
	eaeA	ATGCTTAGTGCTGGTTTAGG GCCTTCATCATTTCGCTTTC	248	94/30	51/30	72/30	Bisi-Johnson et al. (2011)
	hly	AACAAGGATAAGCACTGTTCTGGCT ACCATATAAGCGGTCATTCCCGTCA	1177	94/30	60/50	72/60	Piva et al. (2003)
	stx1	ACACTGGATGATCTCAGTGG CTGAATCCCCCTCCATTATG	614	94/30	58/45	72/45	Dipineto et al. (2006)
	stx2	CCATGACAACGGACAGCAGTT CCTGTCAACTGAGCAGCACTTTG	779	94/30	58/45	72/45	Dipineto et al. (2006)
S. aureus	spa	TCA ACA AAG AAC AAC AAA ATG C GCT TTC GGT GCT TGA GAT TC	226	94/30	5/30	72/30	Wada et al. (2010)
S. aureus	coa	ATAGAGATGCTGGTA CAGG GCT TCC GAT TGT TCG ATG C	430 570 630	94/30	55/40	72/45	Iyerand Kumosani (2011)
P. multocida	toxA	CTTAGATGAGCGACAAGG GAATGCCACACCTCTATAG	864	94/30	48/45	72/45	Tang et al. (2009)