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Table 1 Media features and amino acid content ranges

From: Rich production media as a platform for CHO cell line development

Media classification in this studyClassical media (MEM-a, DMEM, F12, RPMI 1630, etc.)Earlier commercial CHO mediaRecent commercial CHO media
Media characteristicsBasal media used with serum addition or elementary level serum-free media adding some supplementsSerum-free, protein-free media, lean CDMMostly CDM
Years started to be employed1960s–1980s1990s–2000s2000s–2010s
Typical productivityaUp to 0.1 g/LUp to 1 g/L1–10 g/L
Amino acid content rangeb (excluding glutamine)0.5–1 g/L1–3 g/L3–12 g/L
  1. aReferences: Huang et al. (2010) and Takagi et al. (2017)
  2. bFormulation of classical media is publically available. Amino acid content is the expected range based on internal amino acid analysis and/or references (Reinhart et al. 2015; Carrillo-Cocom et al. 2015; Pan et al. 2017). Amino acids are detected (and reported in publications) as molar mass, so the amino acid concentration is calculated with molecular weight