Characterization and antimicrobial activity of lectins purified from three Egyptian leguminous seeds

AMB Express

Table 8 Showed all data (PEAKS 8.5 software) of the complete amino acid sequences of pea lectin (psl)

Peptide Sequences (This study)	− 10lgP	Mass	Length	ppm	m/z	RT	Scan	Intensity sample	Peptide location		PTM
Peptide Sequences (This study)	− 10lgP	Mass	Length	ppm	m/z	RT	Scan	Intensity sample	Start	End	PTM
R.HIGIDVNSIK.S	62.31	1094.61	10	12.0	365.88	15.58	2621	6.72E5	166	175
S.TETTSFLITK.F	40.05	1139.61	10	1.7	570.81	16.93	2848	6.15E3	31	40
R.ALYSSPIHIWDR.E	38.29	1456.75	12	7.1	486.59	19.30	3256	7.71E3	74	85
R.HIGIDVN (+ 0.98) SIK.S	38.07	1095.59	10	10.9	366.21	15.19	2553	1.31E4	166	175	Deamidation (NQ)
K.FSPDQQNLIFQGDGYTTK.E	34.19	2057.97	18	2.4	686.99	18.74	3159	0	41	58
D.RHIGIDVNSIK.S	32.44	1250.71	11	4.1	417.91	14.28	2399	1.55E5	165	175
K.DVVPEWVR.I	30.86	998.52	8	7.2	500.27	18.34	3090	1.13E4	231	238
Total 7 peptides

Peptide sequence of (previous study)	Peptide location (β chain)	References
HIGIDVNSIK	136–145	Sharon and Lis (1990)
TETTSFLITK	1–9
ALYSSPIHIWDR	42–53
FSPDQQNLIFQGDGYTTK	10–27
PEWVR	200–204
DVVPEWVR	83–90	Hemperly et al. (1979)

− 10lgP = Conversion form of P-value in PEAKS 8.5 software where P-value of 1% is equivalent to − 10lgP of 20
ppm delta Mass is the deviation of measured mass from the theoretical mass of the peptide, in ppm, m/z mass of ions (in gas phase) per charge, RT relatively accurate prediction of peptide, PTM post translational modification, NQ variable modification deamidation NQ (+ 0.98 Da) of asparagines (N) and glutamines (Q)