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AMB Express

Table 2 Replacement of various chromosomal regions

From: Systematic approach for assessing whether undeletable chromosomal regions in Saccharomyces cerevisiae are required for cell viability

Name of the region (co-ordinate number)

Replaced sub regions (co-ordinate number)

Number of transformants

Number of transformants analyzed

Transformants with expected replacement

Replaceable/non-replaceable

Chr2-2 (21866–37346)

A1 + A2 (21866–29606)

771

6

6

Replaceable

B1 + B2 (29606–37346)

1001

6

5

Replaceable

A1 + A2 + B1 (21866–33476)

200

6

4

Replaceable

A2 + B1 + B2 (25737–37346)

611

6

6

Replaceable

A1 + A2 + B1 (Ex) (21866–36036)

314

6

4

Replaceable

A2 (Ex) + B1 + B2 (24473–37346)

289

6

6

Replaceable

A1 + A2 + B1 + B2 (21866–37346)

494

6

4

Replaceable

Chr2-6 (318749–330960)

A1 + A2 (318749–324853)

930

6

6

Replaceable

B1 + B2 (324856–330960)

1171

5

5

Replaceable

A1 + A2 + B1 (318749–327908)

411

6

6

Replaceable

A2 + B1 + B2 (321802–330960)

626

6

6

Replaceable

A1 + A2 + B1 (Ex) (318749–329133)

316

6

5

Replaceable

A2 (Ex) + B1 + B2 (321064–330960)

256

6

6

Replaceable

A1 + A2 + B1 + B2 (318749–330960)

769

6

6

Replaceable

Chr9-2 (87850–102249)

A1 + A2 (87850–95050)

731

5

5

Replaceable

B1 + B2 (95050–102249)

703

2

1

Replaceable

A1 + A2 + B1 (87850–98650)

400

6

5

Replaceable

A2 + B1 + B2 (91451–102249)

452

6

3

Replaceable

A1 + A2 + B1 (Ex) (87850–100181)

313

6

6

Replaceable

A2 (Ex) + B1 + B2 (89524–102249)

211

6

6

Replaceable

A1 + A2 + B1 + B2 (87850–102249)

445

6

3

Replaceable

Chr11-2 (188434–204755)

A1 + A2 (188434–196595)

922

6

6

Replaceable

B1 + B2 (196595–204755)

965

6

6

Replaceable

A1 + A2 + B1 (188434–200675)

443

6

2

Replaceable

A2 + B1 + B2 (192515–204755)

627

6

6

Replaceable

A1 + A2 + B1 (Ex) (188434–201328)

364

6

6

Replaceable

A2 (Ex) + B1 + B2 (190334–204755)

217

6

4

Replaceable

A1 + A2 + B1 + B2 (188434–204755)

515

6

4

Replaceable

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