Fig. 7

Checking Chr2-6 (dup + rep) and Chr11-2 (dup + rep) transformants. Upper part of a, b represents DNA module (synthesized from the plasmid pSJ69 as template contained CgLEU2) was introduced into Chr2-6 and Chr11-2 duplicated transformants to replace Chr2-6 and Chr11-2 regions from the intact chromosome, respectively. Bottom part of a, b represents gel electrophoresis of colony PCR and each lane represents independent transformants without the most left lane, which contains size marker. In a, SJP 118 and SJP 119 were used to check left edge of CgLEU2 whereas SJP 127 and SJP 385 were used to check right edge of CgLEU2 for the replacement of Chr2-6 region in Chr2-6 duplicated transformants, respectively. In b, SJP 219 and SJP 119 were used to check left edge of CgLEU2 whereas SJP 127 and SJP 387 were used to check right edge of CgLEU2 for the replacement of Chr11-2 region in Chr11-2 duplicated transformants, respectively