Fig. 6
Molecular detection of T2 plants. aGmVma12 expression level in T2 RNAi silenced transgenic plants by qRT-PCR. b SMV accumulation in SMV-infected T2 transgenic plants by qRT-PCR. Y-axes indicate the transcript levels of GmVma12 (7 dpi) and SMV (7 dpi and 14dpi) of SMV-infected plants. X-axes indicate T2 transgenic plants and non-transformed (Mock) plants. Data are expressed as the means of three biological replicates with error bars indicating the SD (n = 3). Asterisks denote significant difference from mock, as determined by the t-test, p < 0.001. Each result is representative of three biological repeats