Effect of sodium and calcium on polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Lentinus edodes

AMB Express

Table 1 Reaction mixtures of enzyme activity tests

Name of the enzyme	Reaction system	References
UDP-Glcpyrophosphorylase (UGP)	50 mM Tris–HCl buffer (pH7.8), 0.4 mM UDP-Glc, 14 mM MgCl₂,4 U Glc-6-phosphate dehydrogenase, 0.4 mM NADP⁺, 2.1 U α-phospho-glucomutase, 4 mM inorganic pyrophosphate	Bernstein and Robbins (1965)
Phospho-Glcisomerase (PGI)	50 mM potassium phosphate buffer (pH 6.8), 5 mM MgCl₂, 4 U Glc-6-phosphate dehydrogenase, 0.4 mM NADP⁺, 10 mM fructose-6-phosphate	Grobben et al. (1996)
α-Phospho-glucomutase (PGM)	50 mM tri-ethanolamine buffer (pH 7.2), 5 mM MgCl₂, 50 μM Glc-1,6-diphosphate, 0.4 mM NADP⁺, 4 U Glc-6-phosphate dehydrogenase, 1.4 mM Glc-1-phosphate	Qian et al. (1994)