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Table 3 Central composite design of experimental response of four variables (A, cane molasses; B, combined nitrogenous mixture; C, casein hydrolysate; D, glucose)

From: Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium

StdRunFactor (A): cane molasses [% (v/v)]Factor (B): combined nitrogenous mixture (%)aFactor (C): casein hydrolysate (g/L)Factor (D): glucose (g/L)Dry biomass (g/L)
318306.54.511.6
1321052.55311.51
9312453.54.511.8
15412306.54.512.18
24512303.54.511.46
8612303.51.511.03
1278453.51.511.61
28812306.51.512.02
291037.55611.9
17108303.54.510.88
25111037.50311.07
11121037.58312.07
613637.55311.32
23148456.54.511.78
18158303.51.510.27
26161037.55011.65
51712456.54.511.74
14188453.54.511.71
20198456.51.511.86
72012453.51.511.73
10218306.51.511.38
19221037.55312.13
22231022.55310.64
27241037.55312.13
162512456.51.511.91
4261437.55312.06
1271037.55312.11
21281037.55312.15
  1. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00–0.09. All the experiments contained a 1:1:1 mixture of Tween 20, 60 and 80 with a final polysorbate concentration of 0.07%
  2. aThe constant proportions for CSL [% (v/v)], WGE [% (v/v)] and cheese whey [% (w/v)] for preparation of all combined nitrogenous mixtures were 1.35/14.65/1, respectively