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AMB Express

Table 3 Central composite design of experimental response of four variables (A, cane molasses; B, combined nitrogenous mixture; C, casein hydrolysate; D, glucose)

From: Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium

Std

Run

Factor (A): cane molasses [% (v/v)]

Factor (B): combined nitrogenous mixture (%)a

Factor (C): casein hydrolysate (g/L)

Factor (D): glucose (g/L)

Dry biomass (g/L)

3

1

8

30

6.5

4.5

11.6

13

2

10

52.5

5

3

11.51

9

3

12

45

3.5

4.5

11.8

15

4

12

30

6.5

4.5

12.18

24

5

12

30

3.5

4.5

11.46

8

6

12

30

3.5

1.5

11.03

12

7

8

45

3.5

1.5

11.61

28

8

12

30

6.5

1.5

12.02

2

9

10

37.5

5

6

11.9

17

10

8

30

3.5

4.5

10.88

25

11

10

37.5

0

3

11.07

11

12

10

37.5

8

3

12.07

6

13

6

37.5

5

3

11.32

23

14

8

45

6.5

4.5

11.78

18

15

8

30

3.5

1.5

10.27

26

16

10

37.5

5

0

11.65

5

17

12

45

6.5

4.5

11.74

14

18

8

45

3.5

4.5

11.71

20

19

8

45

6.5

1.5

11.86

7

20

12

45

3.5

1.5

11.73

10

21

8

30

6.5

1.5

11.38

19

22

10

37.5

5

3

12.13

22

23

10

22.5

5

3

10.64

27

24

10

37.5

5

3

12.13

16

25

12

45

6.5

1.5

11.91

4

26

14

37.5

5

3

12.06

1

27

10

37.5

5

3

12.11

21

28

10

37.5

5

3

12.15

  1. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00–0.09. All the experiments contained a 1:1:1 mixture of Tween 20, 60 and 80 with a final polysorbate concentration of 0.07%
  2. aThe constant proportions for CSL [% (v/v)], WGE [% (v/v)] and cheese whey [% (w/v)] for preparation of all combined nitrogenous mixtures were 1.35/14.65/1, respectively
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