Fig. 5

TME concentration analysis. To test the activity of the tailor-made enzymes (TMEs) a dilution was prepared and incubated for a 4 h or b 24 h. The enzyme concentrations used were column (1) 187 mg L⁻¹, column (2) 93.6 mg L⁻¹, column (3) 46.8 mg L⁻¹, column (4) 18.7 mg L⁻¹, and column (5) 9.36 mg L⁻¹. The TMEs shown to be active against Schwanniomyces occidentalis at a concentration of 93.6 mg L⁻¹ with a 4 h incubation time, or at a concentration of 18.7 mg L⁻¹ with a 24 h incubation time. However, the best results are obtained when using a TME concentration of 187 mg L⁻¹ and 4 h incubation time, or 46.8 mg L⁻¹ and 24 h incubation time