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Table 1 Effect of various nutritional factors on co-production of various enzymes by A. niger APS

From: A novel multi-enzyme preparation produced from Aspergillus niger using biodegradable waste: a possible option to combat heterogeneous biofilms

Nutritional factors
Carbon Source
CMCaseFPaseβ-GlucosidasePectinaseMannanaseXylanaseGluco-amylaseα-AmylaseAlginate lyase
Enzyme activity (IU/ml)
De-oiled rice bran1.76 ± 0.03*0.687 ± 0.015*0.535 ± 0.0234.11 ± 0.072.31 ± 0.01219.76 ± 0.10527.43 ± 1.421228 ± 19.20.0
Kitchen waste1.08 ± 0.011.24 ± 0.021.02 ± 0.028.52 ± 0.0211.45 ± 0.01219.04 ± 0.2542.67 ± 2.25*2652 ± 31.233.02 ± 0.014
Cellulose1.68 ± 0.030.472 ± 0.020.228 ± 0.022.47 ± 0.0150.00.028.26 ± 0.741160 ± 26.870.0
CMC1.88 ± 0.04*0.811 ± 0.020.647 ± 0.012.56 ± 0.032.3 ± 0.142.4 ± 0.08131.23 ± 0.0321748 ± 18.20.0
Guargum1.72 ± 0.040.604 ± 0.011.77 ± 0.0184.01 ± 0.0175.83 ± 0.0210.023.43 ± 0.761906 ± 24.560.87 ± 0.03
Pectin1.70 ± 0.0141.14 ± 0.0140.849 ± 0.035.8 ± 0.0124.5 ± 0.0180.029.87 ± 1.061416 ± 21.460.834 ± 0.04
Cellobiose1.37 ± 0.0120.803 ± 0.020.122 ± .0172.79 ± 0.0240.43 ± 0.0150.028.28 ± 2.261763 ± 28.260.962 ± 0.06
Lactose1.40 ± 0.040.846 ± 0.020.080 ± 0.0172.38 ± 0.0140.152 ± 0.0210.027.25 ± 1.121461 ± 23.521.26 ± 0.07
Glucose1.32 ± 0.0140.393 ± 0.020.251 ± 0.023.17 ± 0.0170.501 ± 0.0110.020.53 ± 0.982011 ± 38.262.34 ± 0.11*
Galactose1.14 ± 0.040.219 ± 0.020.108 ± 0.0142.32 ± 0.0230.156 ± 0.0120.027.48 ± 1.372139 ± 41.342.16 ± 0.08*
Starch1.49 ± 0.0150.994 ± 0.010.370 ± .0113.67 ± 0.0240.654 ± 0.0181.43 ± 0.0941.48 ± 3.26*2659 ± 36.540.0
Salicin0.89 ± 0.010.370 ± 0.050.108 ± 0.062.47 ± 0.0130.00.04.56 ± 0.042294 ± 6.430.0
Xylose1.53 ± 0.0320.678 ± 0.02*0.939 ± 0.0173.98 ± 0.0140.686 ± 0.0830.59 ± 1.2418.42 ± 1.021288 ± 16.370.926 ± 0.03
Wheat bran + kitchen waste2.61 ± 0.0211.32 ± 0.0132.48 ± 0.0268.36 ± 0.0243.42 ± 0.03640.26 ± 0.4741.33 ± 0.562632 ± 33.03.12 ± 0.04
Control (Wheat bran)2.72 ± 0.020.683 ± 0.061.94 ± 0.035.54 ± 0.243.76 ± 0.0139.05 ± 0.4437.08 ± 0.421574 ± 2.502.16 ± 0.02
Metal ions
 CaSO42.94 ± 0.0141.47 ± 0.0172.89 ± 0.044#10.31 ± 0.071#4.73 ± 0.08643.93 ± 1.0244.02 ± 2.032643 ± 473.42 ± 0.046
 MgSO42.75 ± 0.031.61 ± 0.0222.26 ± 0.01711.26 ± 0.34#6.09 ± 0.1146.27 ± 4.3#43.27 ± 1.872620 ± 532.87 ± 0.011
 K2HPO42.37 ± 0.010.949 ± 0.0632.21 ± 0.0269.72 ± 0.087#5.88 ± 0.12128.18 ± 1.2644.05 ± 2.062094 ± 27#2.63 ± 0.012
 KH2PO42.41 ± 0.210.917 ± 0.042.75 ± 0.01410.26 ± 0.124#4.40 ± 0.9833.85 ± 1.06842.87 ± 1.172691 ± 38.652.54 ± 0.011
 FeSO42.93 ± 0.0221.10 ± 0.0252.70 ± 0.118.31 ± 0.1075.36 ± 1.0340.17 ± 2.9738.86 ± 2.372713 ± 291.87 ± 0.076#
 MnSO42.10 ± 0.14#0.931 ± 0.04#2.17 ± 0.0239.48 ± 0.98#5.51 ± 1.2129.5 ± 1.27#35.54 ± 1.422285 ± 23#1.56 ± 0.011#
 ZnCl21.71 ± 0.023#0.089 ± 0.02#1.16 ± 0.07#8.40 ± 0.0312.35 ± 0.2831.15 ± 0.243#34.23 ± 0.86#1987 ± 21#1.42 ± 0.06#
 KCl1.95 ± 0.012#0.618 ± 0.031#2.40 ± 0.118.36 ± 0.0674.95 ± 0.5620.72 ± 0.87#38.86 ± 1.572485 ± 352.89 ± 0.013
 Control2.64 ± 0.0161.30 ± 0.032.46 ± 0.078.29 ± 0.0833.37 ± 0.01440.85 ± 1.6341.26 ± 0.852612 ± 443.07 ± 0.016
Nitrogen sources
 Ammonium sulphate2.07 ± 0.0120.446 ± 0.032.20 ± 0.014*11.5 ± 0.086*3.18 ± 0.02424.62 ± 2.0333.04 ± 1.4*2944.5 ± 22.581.83 ± 0.014
 Sodium nitrate2.64 ± 0.012*0.920 ± 0.042.18 ± 0.012*11.81 ± 0.065*4.23 ± 0.04225.63 ± 1.8629.42 ± 1.261220 ± 12.361.27 ± 0.018
 Ammonium chloride1.57 ± 0.0260.731 ± 0.031.96 ± 0.02812.26 ± 0.1023.08 ± 0.02822.9 ± 1.7435.49 ± 2.04*3949 ± 56.750.86 ± 0.02
 Urea2.07 ± 0.0140.589 ± 0.021.72 ± 0.0167.98 ± 0.272.79 ± 0.01424.25 ± 1.0530.29 ± 2.852807 ± 23.37*1.13 ± 0.017
 Peptone3.14 ± 0.060*1.22 ± 0.012.25 ± 0.023*11.04 ± 0.1044.13 ± 0.03337.25 ± 1.2445.23 ± 1.22*2692 ± 28.45*2.13 ± 0.011
 Yeast extract2.83 ± 0.02*0.993 ± 0.011.30 ± 0.0678.53 ± 0.47*1.80 ± 0.0432.65 ± 0.6837.75 ± 1.06*2834 ± 18.78*1.75 ± 0.02
 CSL3.79 ± 0.0181.25 ± 0.0123.76 ± 0.02814.72 ± 0.567.72 ± 0.0540.08 ± 2.02*42.27 ± 2.84*2740 ± 37.9*3.17 ± 0.011*
 Soyabean meal1.65 ± 0.0121.26 ± 0.0162.07 ± 0.014*11.09 ± 0.424.67 ± 0.05822.65 ± 1.2837.24 ± 1.06*2932 ± 25.871.83 ± 0.012
 Control2.88 ± 0.0351.56 ± 0.0112.81 ± 0.02210.86 ± 0.0785.23 ± 0.03442.23 ± 1.0241.27 ± 2.032663 ± 31.663.26 ± 0.014
  1. In case of carbon and nitrogen sources, all the values differ from the control significantly (p < 0.001) by Dunnett’s Multiple Comparison Test except those marked with*. In case of metal salts all the values marked with# differ from the control significantly (p < 0.001) by Dunnett’s Multiple Comparison Test